cd45ra allophycocyanin cy7 apc cy7 miltenyi biotec 130 113 363 cd62l r phycoerythrin Search Results


cd45ra allophycocyanin cy7 apc cy7 miltenyi biotec 130 113 363 cd62l r phycoerythrin  (Miltenyi Biotec)
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Miltenyi Biotec cd45ra allophycocyanin cy7 apc cy7 miltenyi biotec 130 113 363 cd62l r phycoerythrin
FIGURE 1 TALEN-mediated multiplex editing generates universal immune evasive CAR T-cells against solid tumor targets. (A) Lentiviral CAR construct for T cell transduction comprised of the anti-tumor targeting single-chain variable fragment (scFv), human CD8a hinge and transmembrane domain, a human 4-1BB costimulatory domain, and a human CD3z activation domain. (B) Experimental strategy for TALEN-mediated gene editing, lentiviral transduction, expansion, and analysis of engineered human universal CAR T-cells. (C) Pictorial representation of immune evasive, universal CAR T- cells engineered with lentiviral CAR expression and TALEN-mediated multiplex editing of TRAC and B2M gene loci, resulting in downregulation of surface TCRa/b and HLA-ABC. (D) Top panel, flow-cytometry plots showing frequency of CAR expression among viable engineered T cells (left), and associated quantitation (right). Bars show the means ± SD, n=3. Bottom panel, flow-cytometry plots showing frequency of TCRa/b (-)/HLA-ABC (-) viable engineered T cells (left) and associated quantitation (right). Bars show means ± SD, n=3. (E) Frequency of FAP UCAR T-cell subpopulations displaying <t>CD62L+CD45RA+</t> (TN naive-like/TSCM memory stem cell), <t>CD62L+CD45RA−(TCM</t> central memory), CD62L-CD45RA−(TEM effector memory) and CD62L-CD45RA+ (TE terminal effector) labeling at end of engineered T cell expansion (n=2). (F) Frequency of FAP UCAR T-cell CD4+
Cd45ra Allophycocyanin Cy7 Apc Cy7 Miltenyi Biotec 130 113 363 Cd62l R Phycoerythrin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 1 TALEN-mediated multiplex editing generates universal immune evasive CAR T-cells against solid tumor targets. (A) Lentiviral CAR construct for T cell transduction comprised of the anti-tumor targeting single-chain variable fragment (scFv), human CD8a hinge and transmembrane domain, a human 4-1BB costimulatory domain, and a human CD3z activation domain. (B) Experimental strategy for TALEN-mediated gene editing, lentiviral transduction, expansion, and analysis of engineered human universal CAR T-cells. (C) Pictorial representation of immune evasive, universal CAR T- cells engineered with lentiviral CAR expression and TALEN-mediated multiplex editing of TRAC and B2M gene loci, resulting in downregulation of surface TCRa/b and HLA-ABC. (D) Top panel, flow-cytometry plots showing frequency of CAR expression among viable engineered T cells (left), and associated quantitation (right). Bars show the means ± SD, n=3. Bottom panel, flow-cytometry plots showing frequency of TCRa/b (-)/HLA-ABC (-) viable engineered T cells (left) and associated quantitation (right). Bars show means ± SD, n=3. (E) Frequency of FAP UCAR T-cell subpopulations displaying CD62L+CD45RA+ (TN naive-like/TSCM memory stem cell), CD62L+CD45RA−(TCM central memory), CD62L-CD45RA−(TEM effector memory) and CD62L-CD45RA+ (TE terminal effector) labeling at end of engineered T cell expansion (n=2). (F) Frequency of FAP UCAR T-cell CD4+

Journal: Frontiers in immunology

Article Title: Stromal depletion by TALEN-edited universal hypoimmunogenic FAP-CAR T cells enables infiltration and anti-tumor cytotoxicity of tumor antigen-targeted CAR-T immunotherapy.

doi: 10.3389/fimmu.2023.1172681

Figure Lengend Snippet: FIGURE 1 TALEN-mediated multiplex editing generates universal immune evasive CAR T-cells against solid tumor targets. (A) Lentiviral CAR construct for T cell transduction comprised of the anti-tumor targeting single-chain variable fragment (scFv), human CD8a hinge and transmembrane domain, a human 4-1BB costimulatory domain, and a human CD3z activation domain. (B) Experimental strategy for TALEN-mediated gene editing, lentiviral transduction, expansion, and analysis of engineered human universal CAR T-cells. (C) Pictorial representation of immune evasive, universal CAR T- cells engineered with lentiviral CAR expression and TALEN-mediated multiplex editing of TRAC and B2M gene loci, resulting in downregulation of surface TCRa/b and HLA-ABC. (D) Top panel, flow-cytometry plots showing frequency of CAR expression among viable engineered T cells (left), and associated quantitation (right). Bars show the means ± SD, n=3. Bottom panel, flow-cytometry plots showing frequency of TCRa/b (-)/HLA-ABC (-) viable engineered T cells (left) and associated quantitation (right). Bars show means ± SD, n=3. (E) Frequency of FAP UCAR T-cell subpopulations displaying CD62L+CD45RA+ (TN naive-like/TSCM memory stem cell), CD62L+CD45RA−(TCM central memory), CD62L-CD45RA−(TEM effector memory) and CD62L-CD45RA+ (TE terminal effector) labeling at end of engineered T cell expansion (n=2). (F) Frequency of FAP UCAR T-cell CD4+

Article Snippet: CD45RA Allophycocyanin-Cy7 (APC-Cy7) Miltenyi Biotec 130-113-363 CD62L R-Phycoerythrin (PE) Miltenyi Biotec 130-113-625 CD4 Phycoerythrin-Vio770 (PE-Vio770) Miltenyi Biotec 130-113-227 CD8 Brilliant Violet 510 BD Bioscience 563919 CD95 Peridinin Chlorophyll Protein-Cy5.5 (PerCPCy5.5) Biolegend 305630 (Continued) frontiersin.org Continued Differentiation

Techniques: Multiplex Assay, Construct, Transduction, Activation Assay, Expressing, Cytometry, Quantitation Assay, Labeling